Literature summary extracted from
Picone, D.; Donnarumma, F.; Ferraro, G.; Russo Krauss, I.; Fagagnini, A.; Gotte, G.; Merlino, A.
Platinated oligomers of bovine pancreatic ribonuclease Structure and stability (2015), J. Inorg. Biochem., 146, 37-43 .
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.6.1.18 |
purified platinated monomeric enzyme, obtained upon RNase A incubation in 1:10 protein to metallodrug ratio, is crystallized at 25°C using the hanging drop vapor diffusion method |
Bos taurus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.18 |
Bos taurus |
P61823 |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.6.1.18 |
More |
the reaction between cis-diamminedichloroplatinum(II), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease, induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A |
Bos taurus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.6.1.18 |
RNase A |
- |
Bos taurus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
4.6.1.18 |
65 |
- |
T1/2 of platinated trimer |
Bos taurus |
4.6.1.18 |
66 |
- |
T1/2 of native enzyme, platinated monomer and platinated dimer |
Bos taurus |